Concentration experiment- For this and every experiment the this procedure was repeated except for some variables specific to each individual experiment. First a cow liver was chopped up to serve as the enzyme of peroxidase. (That procedure was only performed once and used for all three labs to keep the same concentration) Then whatman's #1 filter paper was hole-punched to serve as the disks. Then theses disks were soaked in the enzyme mix, and these were the enzyme soaked disks. Next four disks where placed inside the reaction vessel, and 10 ml. of peroxide was also poured into the vessel. Immediately following, the stopper was used to cap the vessel, however making sure that the peroxide did not come in contact with the enzyme disks. The moment that the stopper was in place the vessel was turned over, placed under the inverted graduated cylinder and the time was recorded for 4 four minutes, recording data at 15 sec intervals.
Temperature experiment-upon beginning the procedure amounts of the enzyme mix was heated to a series of temperatures, all in Celsius; those temperatures being 20, 28, 50, and 65. Then these enzymes where cooled down to room temperature before the procedure above was repeated again.
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